Static electric field inhibits the proliferation and secretion functions of splenic lymphocytes in mice.

In recent years, the effects of static electric field (SEF) generated by ultra-high voltage (UHV) direct current (DC) transmission lines on health has drawn public attention. To study the effects of SEF on the spleen, mice were exposed to 56.3 ± 1.4 kV/m SEF. Results showed that on the 28th day of SEF exposure, the contents of IL-10 and IFN-γ in the supernatant of homogenate, the proliferation levels of lymphocytes and the content of intracellular ROS decreased significantly, while the activity of SOD increased significantly. Meanwhile, the rupture of cellular membrane, the deficiency of mitochondrial cristae and the vacuolization of mitochondria appeared in lymphocytes. Analysis showed that the rupture of the cellular membrane caused the death of T lymphocytes, which would lead to the reduction of IL-10 and IFN-γ secretions. The damage of mitochondria could reduce the production of ATP and the content of ROS, which could inhibit the proliferation of splenic lymphocytes.

Static electric field (SEF) exposure promotes the proliferation of B lymphocytes.

With the rapid development of ultra-high voltage direct current (UHV DC) transmission technology, the intensity of electric fields in the surrounding environment of UHV DC transmission lines significantly increased, which raised public concerns about the potential health effects of electric fields. Previous studies have shown that the exposure of electromagnetic field was associated with cancer. B lymphocytes can produce autoantibodies and tumor growth factors through proliferation, which contributes to the development of cancer. Therefore, this study explored the effect and mechanism of static electric field (SEF) generated by DC transmission lines on the proliferation levels of B lymphocytes. Male mice were exposed to SEF. After the exposure of 7 and 14 days, the proliferation levels of B lymphocytes in the spleens of mice were measured, respectively. To validate biological effect discovered in animal experiments and elucidate the mechanism of the effect from the perspective of signaling pathways, lymphocytes were exposed to SEF. After the exposure of 24, 48 or 72 h, the proliferation levels of B lymphocytes, the expression levels of key proteins and cell cycle were determined. This study found that SEF exposure activated NF-κB pathway by stimulating ERK1/2 pathway and promoted B lymphocytes to enter S phase from G0/G1 phase. Meanwhile, SEF exposure also promoted B lymphocytes to enter G2 phase. Namely, SEF exposure significantly promoted the proliferation of B lymphocytes. This discovery provided theoretical and practical support for the prevention or application of negative or positive effects caused by SEF exposure and provided directions for future research.

The role of Nrf2 in protection against electrostatic field-induced oxidative stress and learning and memory decline in mice.

The intensity of static electric field (SEF) in the surrounding environment of transmission lines has been greatly increased with the rapid development of ultra-high-voltage direct-current transmission. Therefore, the potential health effects of SEF have stimulated great public attention. It has been proven that SEF exposure can cause reversible damage to the nervous system through oxidative stress; however, the mechanism of its recovery is unclear. This study focused on nuclear factor erythroid 2-related factor 2 (Nrf2), a vital regulator of oxidative stress, and has been identified to notably impact the protection of organisms against many external stimuli. Herein, it was found that 56.3 kV/m SEF exposure for 7 days and 14 days significantly improved the expression levels of Nrf2 protein in the cytoplasm and nucleus of mice' hippocampus, as well as antioxidant genes, superoxide dismutase 2, and glutathione peroxidase 1. No significant difference in the expression level of the Nrf2 gene was found. The results indicated that the body could activate the Nrf2 signalling under SEF exposure by means other than up-regulation of Nrf2 gene expression. Inhibiting Nrf2 signalling by isoniazid could block SEF-induced gene transcription and protein expression, resulting in a decrease in antioxidant capacity, an increase in the level of lipid peroxide product, and irretrievability of learning and memory damage. These results demonstrated that the Nrf2 signalling pathway exhibited a protective role in SEF-induced oxidative damage and decline in learning and memory ability, which provides a potential strategy for preventing and treating SEF-related neurotoxicity.

Static electric field exposure decreases white blood cell count in peripheral blood through activating hypothalamic-pituitary-adrenal axis.

With the development of ultra-high-voltage (UHV) direct-current (DC) transmission, the health risk from the static electric field (SEF) generated by UHV DC transmission lines has drawn public attention. To investigate the effect of SEF exposure on white blood cell (WBC) count, mice were exposed to 56.3 kV/m SEF. Results revealed that total WBC count and lymphocyte count significantly decreased and serum levels of corticotropin-releasing hormone, adrenocorticotropic hormone and corticosterone (CORT) significantly increased after the exposure of 7d and 14d. All indices above recovered after the exposure of 21d. Analysis showed that the exposure of 7d and 14d could activate hypothalamic-pituitary-adrenal (HPA) axis. The increased CORT could bind to the glucocorticoid receptor (GR) in lymphocytes, and then promote the migration and apoptosis of lymphocytes. After the exposure of 21d, the magnitude of HPA axis activation declined through CORT-mediated negative feedback and the regulation of stress-related neural circuitry, so WBC count recovered.

Influencing Factors Identification and Prediction of Noise Annoyance-A Case Study on Substation Noise.

Noise-induced annoyance is one person's individual adverse reaction to noise. Noise annoyance is an important basis for determining the acceptability of environmental noise exposure and for formulating environmental noise standards. It is influenced by both acoustic and non-acoustic factors. To identify non-acoustic factors significantly influencing noise annoyance, 40 noise samples with a loudness level of 60-90 phon from 500-1000 kV substations were selected in this study. A total of 246 subjects were recruited randomly. Using the assessment scale of noise annoyance specified by ISO 15666-2021, listening tests were conducted. Meanwhile, basic information and noise sensitivity of each subject were obtained through a questionnaire and the Weinstein's noise sensitivity scale. Based on the five non-acoustic indices which were identified in this study and had a significant influence on noise annoyance, a prediction model of annoyance from substation noise was proposed by a stepwise regression. Results showed that the influence weight of acoustic indices in the model accounted for 80% in which the equivalent continuous A-weighted sound pressure level and the sound pressure level above 1/1 octave band of 125 Hz were 65% and 15%, respectively. The influence weight of non-acoustic indices entering the model was 20% in which age, education level, noise sensitivity, income, and noisy degree in the workplace were 8%, 2%, 4%, 4%, and 2%, respectively. The result of this study can provide a basis for factors identification and prediction of noise annoyance.

Can static electric fields increase the activity of nitric oxide synthase and induce oxidative stress and damage of spleen?

With the rapid development of ultra-high-voltage (UHV) direct-current (DC) transmissions, the impact of static electric fields (SEF) in the vicinity of overhead UHV DC transmission lines on health has aroused much public concern. This study explored the effects of 56.3kV/m SEF on the spleen of mice. Results showed that SEF exposure of 21days significantly increased malonic dialdehyde content, superoxide dismutase activity, calcineurin activity, nitric oxide synthase (NOS) activity, and the mRNA expression levels of tumor necrosis factor-α (TNF-α) and nuclear factor-κB (NF-κB) in the spleen and caused the separation of nucleus and nuclear membrane, the disappearance of mitochondrial membrane, and the deficiency of mitochondrial cristae in splenic lymphocytes. By analysis and discussion, it was deduced that SEF could induce oxidative stress of the spleen by increasing the activity of NOS. Oxidative stress could further cause ultrastructural changes of splenic lymphocytes. Moreover, oxidative stress could cause the increase of the mRNA expression levels of TNF-α and NF-κB, which contributed to the occurrence of spleen inflammation.

Testosterone synthesis in testicular Leydig cells after long-term exposure to a static electric field (SEF).

China's clean energy and resources are mainly located in the west and north while electric load center is concentrated in the middle and east. Thus, these resources and energy need to be converted into electrical energy in situ and transported to electric load center through ultra-high voltage direct current (UHVDC) transmissions. China has built 25,000 km UHVDC transmission lines of 800 kV and 1100 kV, near which the impact of electric field on health has attracted public attention. Previous studies showed that time-varying electromagnetic field exposure could disturb testosterone secretion. To study the effect of non-time-varying electric field caused by direct current transmission lines on testosterone synthesis, male ICR mice were continually (24 h/d) exposed to static electric field of 56.3 ± 1.4 kV/m. Results showed that on the 3rd day of exposure and on the 7th day after ceasing the exposure of 28 d, serum testosterone level and testicular oxidative stress indicators didn't change significantly. On the 28th day of exposure, serum testosterone levels, testicular glutathione peroxidase (GSH-Px) activity, the mRNA and protein levels of testicular StAR, PBR, CYP11A1 decreased significantly, and testicular malondialdehyde (MDA) content increased significantly. Meanwhile, electron-dense edges and vacuolation appeared in lipid droplets of Leydig cells. The gap between inner mitochondrial membrane (IMM) and outer mitochondrial membrane (OMM) enlarged, which would cause the swelling of mitochondria, the rupture and deficiency of mitochondrial membranes. Analysis showed that testicular oxidative stress could induce the damage of mitochondrial structure in Leydig cells, which would decrease the rate of cholesterol transport from cytoplasm to mitochondria. Since cholesterol is the necessary precursor of testosterone synthesis, testosterone synthesis was inhibited. The decrease of the mRNA and protein expression levels of StAR and PBR in testes could diminish the cholesterol transported from OMM to IMM. The decrease of the mRNA and protein expression levels of CYP11A1 could reduce the pregnenolone required in testosterone synthesis and inhibit testosterone synthesis consequently.

Effects of power frequency electric field exposure on kidney.

With the rapid development of ultra high voltage alternating current (UHV AC) transmission, the intensity of environmental power frequency electric field (PFEF) near UHV AC transmission lines increased continuously, which has attracted considerable public attention on the potential health effects of PFEF. In this study, the effect of PFEF exposure on the kidney was explored. Institute of Cancer Research (ICR) mice were exposed to 35 kV/m PFEF (50 Hz). Two indicators relating to renal function (urea nitrogen and creatinine) were tested after the exposure of 7d, 14d, 21d, 35d and 49d. The pathological morphology and cellular ultrastructure of kidney were observed respectively by light microscopy and electron microscopy after the exposure of 25d and 52d. Results showed that compared with that of the control group, the concentration of urea nitrogen of 35 kV/m PFEF exposure group significantly increased on the 21st and 35th days, and the concentration of creatinine significantly increased on the 14th, 21st and 35th days. However, the concentrations of creatinine and urea nitrogen both returned to normal levels on the 49th day. Furthermore, an enlarged Bowman's space, the vacuolation of renal tubular epithelial cells and the foot process effacement of podocyte were found after 25d exposure, but no abnormality was observed after 52d exposure. Obviously, a short-term (35d) exposure of 35 kV/m PFEF could cause kidney injury, which could be recovered after a longer-term (52d) exposure. Based on this study and relevant literatures, one explanation for this two-way effect is as follows. Kidney injury was caused by the disequilibrium of mitochondrial dynamics under 35 kV/m PFEF exposure. PFEF could also activate Wnt/ß-catenin signal to promote the recovery of renal tubular epithelial cells and glomerular podocytes, so kidney injury could be repaired.

Effects of combined traffic noise on the synaptic ultrastructure and expressions of p-CaMKII and NMDAR1 in the hippocampus of young SD rats.

In order to explore the effects of combined traffic noise (CTN) on learning and memory function, young Sprague-Dawley (SD) rats were exposed to CTN from highway and high-speed railway for 52 days, whose day-night equivalent continuous A-weighted sound pressure level (Ldn) was 70 dB(A) (corresponding sound pressure level was 80 dB). The synaptic ultrastructure and the expressions of phosphorylated calcium/calmodulin-dependent protein kinase II (p-CaMKII) and N-methyl-D-aspartate receptor 1 (NMDAR1 or NR1) in the hippocampus were tested by transmission electron microscopy (TEM) and Western blot, respectively. Results showed that there was no significant difference in the synaptic ultrastructure and the expressions of p-CaMKII and NR1 in the hippocampus of young rats between the experimental group and control group. Compared with single high-speed railway noise (HSRN) with Ldn of 70 dB(A), CTN had less influences on learning and memory function, which was closely related to smaller intermittency of CTN and less anxiety caused by CTN. In comparison with white noise with a sound pressure level of 80 dB, CTN had less impacts on learning and memory function, which was mainly associated with CTN's smaller R-weighted sound pressure level based on rats' auditory sensitivity.

A comparative study on influences of static electric field and power frequency electric field on cognition in mice.

Recently, electromagnetic fields around ultra-high voltage transmission lines have received considerable attentions for their potential biological effects. This study aimed to investigate the effects of static electric field (SEF) and power frequency electric field (PFEF) on cognition. Mice were exposed to SEF and PFEF with the same strength (35 kV/m) for 49 days, respectively. Behaviors in Morris water maze test and amino acid neurotransmitter levels in hippocampus were examined during exposure. Results indicated that the exposure of 35 kV/m SEF would not cause significant influences on learning and memory ability in mice, while the exposure of 35 kV/m PFEF would cause significant positive effects on learning and memory ability in mice on day 33. This difference in effects from SEF and PFEF on cognition was possibly induced by the difference in the degree of molecular polarization and ion migration in organisms under exposure of two kinds of electric fields with different frequency.

Studies on effects of static electric field exposure on liver in mice.

With the development of ultra-high-voltage direct-current transmission, the intensity of static electric field (SEF) under transmission lines increased, which has aroused public attention on its potential health effects. In order to examine effects of SEF exposure on liver, institute of cancer research mice were exposed to SEF with intensities of 27.5 kV/m, 34.7 kV/m and 56.3 kV/m, respectively. In each intensity of SEF exposure, a corresponding sham exposure group was used. Several indices relating to liver function (aspartate aminotransferase (AST) and alanine aminotransferase (ALT)) and oxidative stress (superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA)) were tested after exposure of 7, 14, 21 and 35 days. Results showed that exposure to SEF with intensities of 27.5 kV/m and 34.7 kV/m for 35 days did not significantly influence any detected indices above. Under SEF exposure with intensity of 56.3 kV/m, the SOD activity in liver was significantly increased after exposure of 7 and 14 days. However, no significant increase was found in MDA content as well as the activities of AST and ALT between exposure group and sham exposure group during SEF exposure of 56.3 kV/m. It suggested that from three SEF intensities, only exposure to SEF with intensity of 56.3 kV/m (7 and 14 days) caused a temporary oxidative stress response in liver expressed by the increase in activity of SOD, but it did not produce oxidative damage. This biological effect may be related to the increase of mitochondrial membrane potential of hepatocytes caused by SEF exposure. When the membrane potential exceeds a threshold, Q cycle in mitochondria will be affected, which will result in an increase of superoxide anion concentration and ultimately an oxidative stress.

A comparative study on effects of static electric field and power frequency electric field on hematology in mice.

With the development of the ultra high voltage transmission technology, the voltage level of transmission line rised. Accordingly, the strength of electric field in the vicinity of transmission line increased, thus possible health effects from electric field have caused many public attentions. In this study, in order to compare effects induced by static electric field (SEF) and power frequency electric field (PFEF) on immune function, Institute of Cancer Research (ICR) mice were exposed to 35 kV/m SEF (0 Hz) and PFEF (50 Hz),respectively. Several indicators of white blood cell, red blood cell as well as hemoglobin in peripheral blood were tested after exposure of 7, 14 and 21 days, respectively. There was no significant difference in any indicators under SEF exposure of 35 kV/m for 7d, 14d and 21d between experimental group and control group. Under the PFEF exposure of 35 kV/m, white blood cell count significantly reduced after exposure of 7d, 14d and 21d. Meanwhile, red blood cell count significantly reduced after exposure of 7d, and returned to normal level through the compensatory response of organism after exposure of 14d and 21d. Hemoglobin concentration significantly decreased only after exposure of 21d. Based on tested results of hematological indicators, SEF exposure of 35 kV/m did not affect immune functions in mice but PFEF exposure of 35 kV/m could cause a decline of immune function. This difference of effects from SEF and PFEF on immune function was possibly caused by the difference of the degree of molecular polarization and ion migration in organism under exposure of two kinds of electric fields.

Duration-dependent effect of exposure to static electric field on learning and memory ability in mice.

With the rapid development of ultra-high-voltage direct-current (UHVDC) transmission, the strength of environmental static electric field (SEF) around UHVDC transmission lines increased substantially, which has aroused widely public attention on the potential health effects of SEF. In this study, the effect of SEF exposure on learning and memory ability was investigated. Institute of Cancer Research mice were exposed to 56.3 kV/m SEF for a short term (7 days) or long term (49 days). Behaviors in the Morris water maze (MWM) test, hippocampal neurotransmitter contents, and oxidative stress indicators were examined. Results showed that short-term SEF exposure significantly prolonged escape latency and decreased the number of platform-site crossovers, as well as decreased the time spent in the target quadrant in the MWM test. Meanwhile, serotonin level and the ratio of glutamate level to γ-aminobutyric acid level changed significantly. Besides, malondialdehyde content and glutathione peroxidase activity increased significantly, while superoxide dismutase activity decreased significantly. After long-term SEF exposure, all indices above showed no significant differences between the SEF and sham exposure groups. These data indicated that short-term exposure to 56.3 kV/m SEF could cause abnormal neurotransmitter levels and oxidative stress in the hippocampus, which led to the decline in learning and memory ability. Under the condition of long-term exposure, the SEF-induced disturbances in neurotransmitter contents and redox balance were offset by the compensatory responses of mice, and thus, the learning and memory ability returned to normal level. The temporary and reversible decline in learning and memory ability was only a common biological effect of SEF rather than a health hazard.

An Optimization Study on Listening Experiments to Improve the Comparability of Annoyance Ratings of Noise Samples from Different Experimental Sample Sets.

Annoyance ratings obtained from listening experiments are widely used in studies on health effect of environmental noise. In listening experiments, participants usually give the annoyance rating of each noise sample according to its relative annoyance degree among all samples in the experimental sample set if there are no reference sound samples, which leads to poor comparability between experimental results obtained from different experimental sample sets. To solve this problem, this study proposed to add several pink noise samples with certain loudness levels into experimental sample sets as reference sound samples. On this basis, the standard curve between logarithmic mean annoyance and loudness level of pink noise was used to calibrate the experimental results and the calibration procedures were described in detail. Furthermore, as a case study, six different types of noise sample sets were selected to conduct listening experiments using this method to examine the applicability of it. Results showed that the differences in the annoyance ratings of each identical noise sample from different experimental sample sets were markedly decreased after calibration. The determination coefficient (R²) of linear fitting functions between psychoacoustic annoyance (PA) and mean annoyance (MA) of noise samples from different experimental sample sets increased obviously after calibration. The case study indicated that the method above is applicable to calibrating annoyance ratings obtained from different types of noise sample sets. After calibration, the comparability of annoyance ratings of noise samples from different experimental sample sets can be distinctly improved.

Influences of combined traffic noise on the ability of learning and memory in mice.

OBJECTIVE: The present study aimed to evaluate the influences of combined traffic noise (CTN) on the ability of learning and memory in mice. MATERIALS AND METHODS: The Institute of Cancer Research (ICR) mice were exposed to CTN from highways and high-speed railways for 42 days, whose day-night equivalent continuous A-weighted sound pressure level (Ldn) was 70 dB(A). On the basis of behavioral reactions in Morris water maze (MWM) and the concentrations of amino acid neurotransmitters in the hippocampus, the impacts of CTN on learning and memory in mice were examined. RESULTS: The MWM test showed that the ability of learning and memory in mice was improved after short-term exposure (6-10 days, the first batch) to 70 dB(A) CTN, which showed the excitatory effect of stimuli. Long-term exposure (26-30 days, the third batch; 36-40 days, the fourth batch) led to the decline of learning and memory ability, which indicated the inhibitory effect of stimuli. Assays testing amino acid neurotransmitters showed that the glutamate level of the experimental group was higher than that of the control group in the first batch. However, the former was lower than the latter in the third and fourth batches. Both, behavioral reactions and the concentrations of amino acid neurotransmitters, testified that short-term exposure and long-term exposure resulted in excitatory effect and inhibitory effect on the ability of learning and memory, respectively. CONCLUSION: The effects of 70 dB(A) CTN on the ability of learning and memory were closely related to the exposure duration. Furthermore, those effects were regulated and controlled by the level of glutamate in the hippocampus.

Does static electric field from ultra-high voltage direct-current transmission lines affect male reproductive capacity? Evidence from a laboratory study on male mice.

With the development of ultra-high-voltage direct-current (UHVDC) transmission technology and increase in transmission voltage, the issue of environmental static electric field (SEF) pollution is standing out and its possible health effects have caused much public attention. In this study, the effects of chronic exposure to SEF on reproductive capacity of male mice were investigated. Twenty Institute of Cancer Research (ICR) mice were exposed to SEF (56.3 ± 1.4 kV/m, 49 days) generated by a high-voltage device. Several biological end points related to spermatogenesis and testicular function were evaluated, including reproductive organ coefficients, sperm motility and morphology, serum testosterone level, and testicular histology. No significant differences were found between the SEF-exposed and sham-exposed groups at the end of the exposure period. However, further observation through transmission electron microscopy revealed cristae losses in mitochondria of spermatogenic cells after SEF exposure. Nevertheless, the mitochondria injury did not affect sperm motility, which might be explained from the perspective of energy supply. That is, most of the energy required for sperm movement is generated by glycolysis which occurs in the cytoplasm rather than oxidative phosphorylation which occurs in mitochondria. In conclusion, this study indicates that exposure to SEF (56.3 ± 1.4 kV/m, 49 days) has limited effects on male reproductive capacity.

Influences of combined traffic noise on anxiety in mice.

With the rapid development of traffic facilities in China, traffic noise pollution is increasingly prominent. This research aims to explore the influences of combined traffic noise on receptors' anxiety. Institute of cancer research mice were exposed to combined traffic noise (CTN) from highway and high-speed railway for 52days, whose day-night equivalent continuous A-weighted sound pressure level (Ldn) was 70dB(A). The impacts of CTN on anxiety were explored by behavior tests and monoamine neurotransmitter assays. The results were in depth discussed in comparison to two previous studies on the impacts of single high-speed railway noise (HSRN) and aircraft noise (AN), but data from the three studies were not merged and statistically compared. No significant differences were shown in the behavioral indicators and the monoamine levels between the experimental and control groups after CTN exposure, indicating no obvious impacts of 70dB(A) CTN on anxiety in mice were found in this study. When Ldn was approximately 70dB(A), CTN had less obvious impacts on anxiety than HSRN and AN, which is mainly related to that both the acoustical parameters of noise events [maximum noise level (LAmax), noise events duration, slope of rise, difference of LAmax from 1-min background equivalent continuous A-weighted sound pressure level] and modified day-night equivalent continuous R-weighted sound pressure level (considering animal auditory sensitivity to different sound frequencies and circadian rhythms) of CTN are smaller than those of HSRN and AN.

Influence of static electric field on cognition in mice.

With the rapid development of high voltage direct current transmission, the possibility of health effects associated with static electric field (SEF) has caused wide public concern. To examine the effects of long-lasting, full-body exposure to SEF on cognition, Institute of Cancer Research mice were exposed to SEF for 35 d. The intensities of SEF in experimental group I (EG-I), experimental group II (EG-II) and control group (CG) were 2.30∼15.40 kV/m, 9.20∼21.85 kV/m and 0 kV/m, respectively. The performance in learning and memory of mice were tested by Morris water maze (MWM) on days 2∼6, 16∼20 and 30∼34 during the exposure period. The concentrations of hippocampal amino acid neurotransmitters were evaluated on days 7, 21 and 35. Results showed that the latency in the MWM test had no significant difference among the EG-I, EG-II and CG (P > 0.05) during the exposure period. The percentage of time spent in the target quadrant was significantly decreased in the EG-II on day 34 during the exposure period (P < 0.05), whereas the percentage of time spent in the opposite quadrant increased markedly (P < 0.01). The glutamate and gamma-aminobutyric acid concentrations showed no significant differences among the EG-I, EG-II and CG (P > 0.05) during the exposure period. These results indicated that long-lasting, full-body exposure to SEF with certain intensity would not cause significant influence on learning ability, but it might associate with memory impairment of receptors. Meanwhile, this effect of memory impairment was dose-dependent and not causally linked to the glutamate and gamma-aminobutyric acid levels in the hippocampus.

Emotion recognition from sound stimuli based on back-propagation neural networks and electroencephalograms.

This research aims to explore the feasibility of using back-propagation (BP) neural networks and electroencephalograms (EEGs) to recognize the emotional reactions induced by sound stimuli in the dimensions of pleasure and arousal, as well as compare the recognition performance of each method on these two dimensions. It could provide an aided design on choosing proper sounds to induce or regulate individuals' emotional states under specific situations for potential users at the design stage. Emotional reactions to different sound stimuli are investigated by Self-Assessment Manikin. The results of BP neural network indicate that the arousal predictions are more satisfactory than the pleasure predictions, and the recognition rates can be improved by optimizing input parameters. EEG signals induced by sound stimuli are recorded. The results show that when induced by each pleasant sound, the Average Power of Electroencephalogram of the α wave in the left frontal pole electrode is significantly lower than that in the right frontal pole electrode, while when induced by each unpleasant sound, the former is significantly higher than the latter. This finding indicates that pleasant and unpleasant sounds can be identified based on the asymmetry of the α wave between the left and right frontal pole electrodes.

Annoyance and activity disturbance induced by high-speed railway and conventional railway noise: a contrastive case study.

BACKGROUND: High-speed railway (HR, Electrified railway with service speed above 200 km/h.) noise and conventional railway (CR, Electrified railway with service speed under 200 km/h.) noise are different in both time and frequency domain. There is an urgent need to study the influence of HR noise and consequently, develop appropriate noise evaluation index and limits for the total railway noise including HR and CR noise. METHODS: Based on binaural recording of HR and CR noises in a approximate semi-free field, noise annoyance and activity disturbance induced by maximal train pass-by events in China were investigated through laboratory subjective evaluation. 80 students within recruited 102 students, 40 males and 40 females, 23.9 ± 2.1 years old, were finally selected as the subjects. After receiving noise stimulus via headphone of a binaural audio playback system, subjects were asked to express the annoyance or activity disturbance due to railway noise at a 0-100 numerical scale. RESULTS: The results show that with the same annoyance rating (A) or activity disturbance rating (D), the A-weighted equivalent sound pressure level (LAeq) of CR noise is approximately 7 dB higher than that of HR noise. Linear regression analysis between some acoustical parameters and A (or D) suggests that the coefficient of determination (R2) is higher with the instantaneous fast A-weighted sound pressure level (LAFmax) than that with LAeq. A combined acoustical parameter, LHC = 1.74LAFmax + 0.008LAFmax(Lp-LAeq), where Lp is the sound pressure level, was derived consequently, which could better evaluate the total railway noise, including HR and CR noise. More importantly, with a given LHC, the noise annoyance of HR and CR noise is the same. CONCLUSIONS: Among various acoustical parameters including LHC and LAeq, A and D have the highest correlation with LHC. LHC has been proved to be an appropriate index to evaluate the total railway noise, including both HR and CR. However, it should be pointed out that this study provides suggestive evidence, rather than a final proof. Further study is expected to elucidate conclusions above by additional measurements.